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mouse transam irf3 kit  (Active Motif)

 
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    Active Motif mouse transam irf3 kit
    MØ from C57BL-6 WT mice were cultured for 1h, 2h and 4h in medium alone, or with P. gingivalis strain 381 (Pg) at MOI 100. One hour PolyI:C (1μg/ml) treatment was used as a positive control for <t>IRF3</t> activation. MØ cultured in medium alone (open bars), and MØ cultured with P. gingivalis strain 381 (Pg) or PolyI:C (filled bars). MØ IRF3 activation was measured in nuclear fractions (A), or cytoplasmic fractions (B) by spectrophotometric assay per manufacturer’s instructions. Data are presented as mean ± SEM of two independent experiments (n = 1 mouse per experiment).
    Mouse Transam Irf3 Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse transam irf3 kit/product/Active Motif
    Average 90 stars, based on 1 article reviews
    mouse transam irf3 kit - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3"

    Article Title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3

    Journal: Innate immunity

    doi: 10.1177/1753425913492180

    MØ from C57BL-6 WT mice were cultured for 1h, 2h and 4h in medium alone, or with P. gingivalis strain 381 (Pg) at MOI 100. One hour PolyI:C (1μg/ml) treatment was used as a positive control for IRF3 activation. MØ cultured in medium alone (open bars), and MØ cultured with P. gingivalis strain 381 (Pg) or PolyI:C (filled bars). MØ IRF3 activation was measured in nuclear fractions (A), or cytoplasmic fractions (B) by spectrophotometric assay per manufacturer’s instructions. Data are presented as mean ± SEM of two independent experiments (n = 1 mouse per experiment).
    Figure Legend Snippet: MØ from C57BL-6 WT mice were cultured for 1h, 2h and 4h in medium alone, or with P. gingivalis strain 381 (Pg) at MOI 100. One hour PolyI:C (1μg/ml) treatment was used as a positive control for IRF3 activation. MØ cultured in medium alone (open bars), and MØ cultured with P. gingivalis strain 381 (Pg) or PolyI:C (filled bars). MØ IRF3 activation was measured in nuclear fractions (A), or cytoplasmic fractions (B) by spectrophotometric assay per manufacturer’s instructions. Data are presented as mean ± SEM of two independent experiments (n = 1 mouse per experiment).

    Techniques Used: Cell Culture, Positive Control, Activation Assay, Spectrophotometric Assay

    MØ from C57BL-6 WT, IRF3−/− (A), TRIF−/− (B), TLR4−/− (C) and MyD88−/− (D) mice were cultured for 24h in medium alone (open bars) or with P. gingivalis strain 381 at MOI 100 (filled bars). ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 3–6 individual mice per group in total. * = P<0.05 as determined by unpaired t-test.
    Figure Legend Snippet: MØ from C57BL-6 WT, IRF3−/− (A), TRIF−/− (B), TLR4−/− (C) and MyD88−/− (D) mice were cultured for 24h in medium alone (open bars) or with P. gingivalis strain 381 at MOI 100 (filled bars). ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 3–6 individual mice per group in total. * = P<0.05 as determined by unpaired t-test.

    Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay

    MØ from C57BL-6 WT (open bars), and IRF3−/− (filled bars) mice were cultured for 24h with P. gingivalis (Pg) strain 381 and P. gingivalis strain 33277 at MOI 100. ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 4 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test.
    Figure Legend Snippet: MØ from C57BL-6 WT (open bars), and IRF3−/− (filled bars) mice were cultured for 24h with P. gingivalis (Pg) strain 381 and P. gingivalis strain 33277 at MOI 100. ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 4 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test.

    Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay

    MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing P. gingivalis 381 at MOI 100, 10, and 1 for 24h. ELISA was used to measure TNF-α (A), IL-6 (B), and RANTES (C) in culture supernatant fluids. For ligand treatments, MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing ultra pure E. coli 0111:B4 LPS (Ec-LPS, 10 ng/ml) or medium containing ultra pure Pam3CSK4 (100 ng/ml) for 24h. TNF-α levels in culture supernatant fluids was measured by ELISA (D). Bar represents mean ± SEM from two separate experiments; n = 3–5 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test. ND = None detected; NS = Not significant.
    Figure Legend Snippet: MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing P. gingivalis 381 at MOI 100, 10, and 1 for 24h. ELISA was used to measure TNF-α (A), IL-6 (B), and RANTES (C) in culture supernatant fluids. For ligand treatments, MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing ultra pure E. coli 0111:B4 LPS (Ec-LPS, 10 ng/ml) or medium containing ultra pure Pam3CSK4 (100 ng/ml) for 24h. TNF-α levels in culture supernatant fluids was measured by ELISA (D). Bar represents mean ± SEM from two separate experiments; n = 3–5 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test. ND = None detected; NS = Not significant.

    Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay

    Viability of MØ from WT and IRF3−/− mice in response to P. gingivalis
    Figure Legend Snippet: Viability of MØ from WT and IRF3−/− mice in response to P. gingivalis

    Techniques Used:

    MØ from C57BL-6 WT (open bars), IRF3−/− (black filled bars), and IRF7−/− (gray filled bars) mice were cultured for 6h (A) or 24h (B) with P. gingivalis strain 381 at MOI 100. Cell culture supernatant fluids were harvested and ELISA was used to measure TNF-α levels. Data presented as a percentage of WT levels from two separate experiments; n = 6 individual mice per group in total. * = P<0.05 by one-way ANOVA with Tukey’s post test. NS = not significant.
    Figure Legend Snippet: MØ from C57BL-6 WT (open bars), IRF3−/− (black filled bars), and IRF7−/− (gray filled bars) mice were cultured for 6h (A) or 24h (B) with P. gingivalis strain 381 at MOI 100. Cell culture supernatant fluids were harvested and ELISA was used to measure TNF-α levels. Data presented as a percentage of WT levels from two separate experiments; n = 6 individual mice per group in total. * = P<0.05 by one-way ANOVA with Tukey’s post test. NS = not significant.

    Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay



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    mouse transam irf3 kit  (Active Motif)
    90
    Active Motif mouse transam irf3 kit
    MØ from C57BL-6 WT mice were cultured for 1h, 2h and 4h in medium alone, or with P. gingivalis strain 381 (Pg) at MOI 100. One hour PolyI:C (1μg/ml) treatment was used as a positive control for <t>IRF3</t> activation. MØ cultured in medium alone (open bars), and MØ cultured with P. gingivalis strain 381 (Pg) or PolyI:C (filled bars). MØ IRF3 activation was measured in nuclear fractions (A), or cytoplasmic fractions (B) by spectrophotometric assay per manufacturer’s instructions. Data are presented as mean ± SEM of two independent experiments (n = 1 mouse per experiment).
    Mouse Transam Irf3 Kit, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse transam irf3 kit/product/Active Motif
    Average 90 stars, based on 1 article reviews
    mouse transam irf3 kit - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    MØ from C57BL-6 WT mice were cultured for 1h, 2h and 4h in medium alone, or with P. gingivalis strain 381 (Pg) at MOI 100. One hour PolyI:C (1μg/ml) treatment was used as a positive control for IRF3 activation. MØ cultured in medium alone (open bars), and MØ cultured with P. gingivalis strain 381 (Pg) or PolyI:C (filled bars). MØ IRF3 activation was measured in nuclear fractions (A), or cytoplasmic fractions (B) by spectrophotometric assay per manufacturer’s instructions. Data are presented as mean ± SEM of two independent experiments (n = 1 mouse per experiment).

    Journal: Innate immunity

    Article Title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3

    doi: 10.1177/1753425913492180

    Figure Lengend Snippet: MØ from C57BL-6 WT mice were cultured for 1h, 2h and 4h in medium alone, or with P. gingivalis strain 381 (Pg) at MOI 100. One hour PolyI:C (1μg/ml) treatment was used as a positive control for IRF3 activation. MØ cultured in medium alone (open bars), and MØ cultured with P. gingivalis strain 381 (Pg) or PolyI:C (filled bars). MØ IRF3 activation was measured in nuclear fractions (A), or cytoplasmic fractions (B) by spectrophotometric assay per manufacturer’s instructions. Data are presented as mean ± SEM of two independent experiments (n = 1 mouse per experiment).

    Article Snippet: Levels of IRF3 in cytoplasmic and nuclear fractions were determined on 7μg of total protein, and presented as arbitrary units at 450 nm using mouse TransAM IRF3 kit according to manufacturer’s instructions (Active Motif).

    Techniques: Cell Culture, Positive Control, Activation Assay, Spectrophotometric Assay

    MØ from C57BL-6 WT, IRF3−/− (A), TRIF−/− (B), TLR4−/− (C) and MyD88−/− (D) mice were cultured for 24h in medium alone (open bars) or with P. gingivalis strain 381 at MOI 100 (filled bars). ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 3–6 individual mice per group in total. * = P<0.05 as determined by unpaired t-test.

    Journal: Innate immunity

    Article Title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3

    doi: 10.1177/1753425913492180

    Figure Lengend Snippet: MØ from C57BL-6 WT, IRF3−/− (A), TRIF−/− (B), TLR4−/− (C) and MyD88−/− (D) mice were cultured for 24h in medium alone (open bars) or with P. gingivalis strain 381 at MOI 100 (filled bars). ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 3–6 individual mice per group in total. * = P<0.05 as determined by unpaired t-test.

    Article Snippet: Levels of IRF3 in cytoplasmic and nuclear fractions were determined on 7μg of total protein, and presented as arbitrary units at 450 nm using mouse TransAM IRF3 kit according to manufacturer’s instructions (Active Motif).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    MØ from C57BL-6 WT (open bars), and IRF3−/− (filled bars) mice were cultured for 24h with P. gingivalis (Pg) strain 381 and P. gingivalis strain 33277 at MOI 100. ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 4 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test.

    Journal: Innate immunity

    Article Title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3

    doi: 10.1177/1753425913492180

    Figure Lengend Snippet: MØ from C57BL-6 WT (open bars), and IRF3−/− (filled bars) mice were cultured for 24h with P. gingivalis (Pg) strain 381 and P. gingivalis strain 33277 at MOI 100. ELISA was used to measure TNF-α levels in harvested culture supernatant fluids. Data are presented as mean ± SEM from two separate experiments; n = 4 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test.

    Article Snippet: Levels of IRF3 in cytoplasmic and nuclear fractions were determined on 7μg of total protein, and presented as arbitrary units at 450 nm using mouse TransAM IRF3 kit according to manufacturer’s instructions (Active Motif).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing P. gingivalis 381 at MOI 100, 10, and 1 for 24h. ELISA was used to measure TNF-α (A), IL-6 (B), and RANTES (C) in culture supernatant fluids. For ligand treatments, MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing ultra pure E. coli 0111:B4 LPS (Ec-LPS, 10 ng/ml) or medium containing ultra pure Pam3CSK4 (100 ng/ml) for 24h. TNF-α levels in culture supernatant fluids was measured by ELISA (D). Bar represents mean ± SEM from two separate experiments; n = 3–5 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test. ND = None detected; NS = Not significant.

    Journal: Innate immunity

    Article Title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3

    doi: 10.1177/1753425913492180

    Figure Lengend Snippet: MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing P. gingivalis 381 at MOI 100, 10, and 1 for 24h. ELISA was used to measure TNF-α (A), IL-6 (B), and RANTES (C) in culture supernatant fluids. For ligand treatments, MØ from C57BL-6 WT (open bars) and IRF3−/− (filled bars) mice were cultured with medium alone (Cont), medium containing ultra pure E. coli 0111:B4 LPS (Ec-LPS, 10 ng/ml) or medium containing ultra pure Pam3CSK4 (100 ng/ml) for 24h. TNF-α levels in culture supernatant fluids was measured by ELISA (D). Bar represents mean ± SEM from two separate experiments; n = 3–5 individual mice per group in total. * = P<0.05 by two-way ANOVA with Bonferroni post test. ND = None detected; NS = Not significant.

    Article Snippet: Levels of IRF3 in cytoplasmic and nuclear fractions were determined on 7μg of total protein, and presented as arbitrary units at 450 nm using mouse TransAM IRF3 kit according to manufacturer’s instructions (Active Motif).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    Viability of MØ from WT and IRF3−/− mice in response to P. gingivalis

    Journal: Innate immunity

    Article Title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3

    doi: 10.1177/1753425913492180

    Figure Lengend Snippet: Viability of MØ from WT and IRF3−/− mice in response to P. gingivalis

    Article Snippet: Levels of IRF3 in cytoplasmic and nuclear fractions were determined on 7μg of total protein, and presented as arbitrary units at 450 nm using mouse TransAM IRF3 kit according to manufacturer’s instructions (Active Motif).

    Techniques:

    MØ from C57BL-6 WT (open bars), IRF3−/− (black filled bars), and IRF7−/− (gray filled bars) mice were cultured for 6h (A) or 24h (B) with P. gingivalis strain 381 at MOI 100. Cell culture supernatant fluids were harvested and ELISA was used to measure TNF-α levels. Data presented as a percentage of WT levels from two separate experiments; n = 6 individual mice per group in total. * = P<0.05 by one-way ANOVA with Tukey’s post test. NS = not significant.

    Journal: Innate immunity

    Article Title: Inflammatory response to Porphyromonas gingivalis partially requires interferon regulatory factor (IRF) 3

    doi: 10.1177/1753425913492180

    Figure Lengend Snippet: MØ from C57BL-6 WT (open bars), IRF3−/− (black filled bars), and IRF7−/− (gray filled bars) mice were cultured for 6h (A) or 24h (B) with P. gingivalis strain 381 at MOI 100. Cell culture supernatant fluids were harvested and ELISA was used to measure TNF-α levels. Data presented as a percentage of WT levels from two separate experiments; n = 6 individual mice per group in total. * = P<0.05 by one-way ANOVA with Tukey’s post test. NS = not significant.

    Article Snippet: Levels of IRF3 in cytoplasmic and nuclear fractions were determined on 7μg of total protein, and presented as arbitrary units at 450 nm using mouse TransAM IRF3 kit according to manufacturer’s instructions (Active Motif).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay